Xenbase Image ID: 158910

Figure 4. Homology of Xenopus and mouse ATM development.(A) FACS analysis of mouse SCs that were isolated from iWAT at postnatal day (PD) 7. Representative of 3 independent assays. Details of FACS analysis of ATMs have been described previously [19]. (B) TEM images of sorted cells of the iWAT stroma at PD7; also see Supplemental Fig. 4. Scale bar, 10 μm. (C) Identification of ATMs on the basis of CD11b, F4/80 antigen, and lysosomal protein Mac-3/Lamp-2 expression. Cells were isolated from iWAT at PD7. (D and E) Characterization of ATMs of the iWAT at PD7. (F) Immunohistochemistry against CX3CR1 in iWAT at PD7. F-Actin was labeled with fluorescent phalloidin. (G) Percentage of CX3CR1+ ATMs in the iWAT at PD7 and PD56. (H) Scheme for labeling YS-derived Mϕs in the mouse. (I) Percentage of YS-derived (CX3CR1-eGFP+) ATMs in wild-type (WT) and transgenic (TG) iWAT at PD7 (Flow Repository accession number FR-FCM-ZYZW). (J) Percentage of Ki-67+ ATMs in iWAT at PD7 and PD56, pooled data from 3 assays, and amount of CD45.2+ leukocytes in BM and blood and epididymal WAT ATMs, 20 wk after BM transplantation (n = 3; Flow Repository accession number FR-FCM-ZYZW). (K and L) Scheme of the proposed homology of ATM development in Xenopus and mouse. FSC, forward scatter; iMC = immature myeloid cell, lp = lamellipoda, nc = nucleus, ns = non-significant, POD = peroxidase, SSC = side scatter, vs = vesicles. ** P<0.01, ANOVA with Dunnett’s post-hoc test. Image published in: Hassnain Waqas SF et al. (2017) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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