Xenbase Image ID: 158911

Figure 5. Control of ATM number and activation state by gpr147 and NPFF.(A) Relative transcription of gpr147 mRNA in ATMs, adipocytes (ACs), and various tissues (n = 4). Transcript levels are normalized to ATMs. Insert shows predicted primary structure of Xenopus gpr147 using LOMETS structure prediction software [84]. (B) Transcription of lurp in wild-type (WT) and gpr147-KO fat body (n = 5). (C) ACP+ cells (arrowhead) in fat body of WT and gpr147-KO adult frog. Scale bar, 50 μm. (D) Number of ATMs that were isolated from WT and gpr147-KO fat bodies of adult frogs (n = 5). (E) Representative images of SCs of a WT frog cultured in soft agar and treated with vehicle or 0.5 nM NPFF. Colonies were photographed at day 3 of treatment. (F) Size of vehicle- or NPFF-treated colonies (n > 80 each assay), pooled data of 3 assays. (G) Relative transcription of Mϕ activation genes in ATMs of WT and gpr147-KO frogs (n = 5). (H) Xenopus ATMs were treated with vehicle or 1 nM NPFF for 1 h in vitro and relative transcription of Mϕ activation genes was measured (n = 3). (I) Relative transcription of rnf128 in WT and npffr1-KO ATMs (n = 5), and in ATMs treated in vitro with vehicle or 1 nM NPFF for 1 h (n = 3). (J) Relative transcription of Mϕ activation genes in ATMs of in vitro cultured Xenopus ATMs, treated with vehicle, 1 nM NPFF, pI:pC, or combination of pI:pC and NPFF for 4 h (n = 3). ac = adipocyte. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t test (B–I), or 1-way ANOVA with Dunnett’s post hoc test (A and J). Image published in: Hassnain Waqas SF et al. (2017) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Larger Image Printer Friendly View

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