Xenbase Image ID: 158912

Figure 1. ZC4H2 is required for neural development in Xenopus. (a) RT-PCR analysis of the developmental expression of ZC4H2 in Xenopus. H4 is an internal control. NC, negative control without reverse transcriptase in the RT reaction. (b–g) The expression pattern of ZC4H2 revealed by in situ hybridization at cleavage embryos (b,c lateral view), gastrula embryos (d, ventral view), stage 15 (e, dorsal view) and stage 25 (f, lateral view) Xenopus embryos. (g) Paraffin sections of the embryos shown in (e) showed the expression of ZC4H2 at neural tubes. (h) Morphology of tadpoles (stage 42) injected with standard MO (25 ng), ZC4H2 MO (25 ng) and ZC4H2 MO (25 ng) with ZC4H2 mRNA (0.6 ng). Embryos were injected in both blastomeres at the two-cell stage and raised to the indicated tadpole stage. (i) Statistics of the data shown in panel (h). Con., control, Res., rescue. (j) ZC4H2 MO (25 ng) with or without ZC4H2 mRNA (0.6 ng) was injected into one cell of four-cell-stage embryos, and whole-mount in situ hybridization with probes of Sox2 and Slug was processed at stage 14–16. GFP mRNA was co-injected to trace the injected sides (on the right sides). i.s., injected side. (k) Statistics of the data shown in panel (j). (l) RT-PCR analysis in animal cap assay showing the effect of ZC4H2 on the expression of the genes of Pax3, Sox2, Slug and Keratin. Embryos were injected in both blastomeres at the two-cell stage and the animal cap tissues were dissected at stage 8 and then were cultured for 20–24 h before harvesting. RT, negative control with reverse-transcriptase omitted in the RT reaction; embryos, RNA template from whole embryos was used as a positive control. H4 is an internal control. Image published in: Ma P et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue zc4h2.L hca127, KIAA1166 X. laevis Throughout NF stage 3 (4-cell) animal hemisphere zc4h2.L hca127, KIAA1166 X. laevis Throughout NF stage 10.5 dorsal marginal zone neuroectoderm zc4h2.L hca127, KIAA1166 X. laevis Sometime during NF stage 17 to NF stage 18 neuroectoderm neural plate zc4h2.L hca127, KIAA1166 X. laevis Throughout NF stage 27 neural tube posterior neural tube anterior neural tube brain interneuron Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + zc4h2 MO + NF42 (whole-mount microscopy) Fig. 1 h. Anatomical Phenotype abnormal eye abnormal head abnormal whole organism abnormally decreased number of pigment cell decreased length of anterior-posterior axis decreased length of trunk Xla Wt + zc4h2 MO + NF18 (whole-mount microscopy) Fig. 1 j Expression Phenotype decreased amount snai2.L expression in neural plate border increased amount sox2.L expression in neural plate Xla Wt + zc4h2 MO + NF18 (Other Detection Assay) Fig. 1 l Expression Phenotype decreased amount krt12.4.L expression in epidermis increased amount pax3.L expression in neural plate decreased amount snai2.L expression in neural plate border increased amount sox2.S expression in neural plate Larger Image Printer Friendly View

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