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Figure 3. ZC4H2 inhibits myogenic differentiation of C2C12 cells. (a–c) mRNA levels of ZC4H2, myogenic markers MyHC and Myf5 during myogenic induction in C2C12 cells with ZC4H2 over-expressed. C2C12 cells were firstly transfected with the indicated plasmids 8 h before myogenic induction in DMEM with the addition of 2% horse serum for 2 days. After induction, total RNAs were isolated and analysed by real-time PCR. (d–f) mRNA levels of ZC4H2, myogenic markers MyHC and Myf5 during myogenic induction when ZC4H2 was knocked-down. C2C12 cells were transfected with the indicated siRNAs and then treated with the induction medium for 2 days. The experiments were carried out as described for panel (a–c). n.s., not significant; **p < 0.01. NC, negative control. Image published in: Ma P et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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