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Figure 6. ZC4H2 interacts with Smad1/5. (a,b) ZC4H2 was co-immunoprecipitated with Smad1 (a) or Smad5 (b). (c,d) Smad1 (c) or Smad5 (d) was coimmunoprecipitated with ZC4H2. HEK293 cells were transiently transfected with different combinations of expression vectors of ZC4H2 and Smad1/5, as indicated. Cell lysates were incubated with anti-FLAG beads, washed, and subsequently analysed by western blotting. (e) Schematic of the structures of Smad1/5 truncates. (f,g) The interaction between ZC4H2 and Smad1 (f) or Smad5 (g) different truncations. HEK293 cells were transiently transfected with different combinations of expression vectors of myc-tagged ZC4H2 and FLAG-tagged various Smad1 (e) or Smad5 (f) truncates, as indicated. Cell lysates were incubated with anti-FLAG beads and subsequently analysed by western blotting. WCL, whole-cell lysate; IB, immunoblot; IP, immunoprecipitation. Image published in: Ma P et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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