XB-IMG-158934
Xenbase Image ID: 158934
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Figure 2. Expression of Xenopus Follistatin (XF-319) mRNAs during Different Stages of Xenopus Development
(A) Developmental Northern blot using 2 pg of stage-specific poly(A)+ RNA per lane. The blot was probed with full-length XFS-319 cDNA. Two
transcripts of 3.6 and 2.4 kb are detected beginning at late gastrula/early neurula (stage 12). Overexposure of the same blot indicates that the
2.4 kb transcript is present maternally (data not shown). The embryonic stages are as follows: 9, blastula; IO, 11, and 12 are early, middle, and
late gastrula; 13, 14, and 20 are early, middle, and late neurula; 36 is a tadpole stage. The lower panel shows the hybridization of the same blot
with the Xenopus fibronectin gene to test for RNA recovery. The low follistatin and fibronectin signals at stage 11 appear to be due to the fact
that less RNA is loaded in that lane (see whole-mount in situ hybridizations for stage 11 in Figure 38).
(B) XFS-319 transcript is expressed maternally. RT-PCR analysis of total RNA from fertilized eggs demonstrates that the XFS-319 transcript is
encoded maternally. The control lane is a reaction with all the ingredients of the neurula lane taken through the whole procedure, from which,
however, the RT was omitted. The presence of the translational elongation factor, EF-la, is used as positive control.
(C) XFS-319 RNA is present on the dorsal side at the onset of gastrulation. Embryos at stage 10% were dissected into dorsal and ventral parts.
RNA from each part was analyzed by RT-PCR. XFS-319 is present only in the lane containing RNA from the dorsal side. The presence of noggin
and goosecoid in the same fraction controls for the accuracy of the dissections. The assay for EF-la demonstrates again that comparable amounts
of RNA are present in all experimental lanes. The control lane contains all the ingredients of the embryo lanes except for RT. Image published in: Hemmati-Brivanlou A et al. (1994) Copyright © 1994. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |