Xenbase Image ID: 158939

Figure 7. XFS-319 Does Not Induce the Expression of Immediate Early Mesodermal Markers in Animal Cap Explants Embryos were injected in the animal pole of both blastomeres with 2 ng of XFS-319 RNA or control globin RNA. Animal caps, from uninjetted and injected embryos, were explanted at blastula stage (stage 8) and divided into two groups. The first group was allowed to develop until sibling uninjected embryos reached midgastrula stage (stage 10.5), and RT-PCR assay was used to score for expression of immediate- early mesodermal markers in uninjected or injected animal caps. Neither of the two general immediate early markers of mesoderm, X&a and MM, nor the dorsal specific markers, noggin and goosecoid, nor the ventral marker Xwnf-8 are induced by XFS-319. Addition of activin to the uninjected caps induced the expression of all of these markers in the explants, demonstrating that these caps are responsive to induction. The embryo lane represents RNA extracted from stage 10.5 embryos and provides a positive control for the primers and the size of the expected product. The negative control lane contains all the ingredients of the embryo lane except for RT. The second group was cultured until early tailbud stages and analyzed by RT-PCR for expression of neural markers (as in Figure 9). This provides a positive control for the neural inducing activity of XFS-319 RNA. Image published in: Hemmati-Brivanlou A et al. (1994) Copyright © 1994. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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