XB-IMG-158940
Xenbase Image ID: 158940
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Figure 8. Dose Response Effect of XFS-319 RNA and Neural Induction
Embryos were injected in the animal pole at the 2-cell stage, in both
blastomeres, with different concentrations of XFS-319 RNA. Animal
caps were explanted from blastulae (stage 8) and cultured until early
tailbud stage (stage 28) at which point total RNA was harvested from
all explants and analyzed by Northern blots for the expression of the
general neural marker, f&tubulin isotype II, as well as the cement gland
marker, XAG-7. While the uninjected control animal cap does not show
expression of either marker, as little as 50 pg of XFS-319 induces the
general neural marker. Moreover, the cement gland-specific marker
requires a minimum of 250 pg to be induced. The same blot was
stripped of its signals and reprobed with muscle actin. This control
demonstrates that neuralization is direct and was not accompanied
by mesoderm induction. The two top ubiquitous bands of actin demonstrate
that comparable amounts of RNA were loaded in each lane. Image published in: Hemmati-Brivanlou A et al. (1994) Copyright © 1994. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |