Xenbase Image ID: 158952

Fig. S8. Schematic of morpholino injection into X. tropicalis and summary of resulting vascular disruptions. A) Rasip1-MO and Alexa488 lineage tracer are co-injected at 8 ng/cell into two blastomeres of 4–8 cell stage embryos. Embryos are allowed to develop to desired stage: st.20–21 to assess angioblast specification, st.25–27 to assess vasculogenesis in progress, or st.33–35 to assess vessels following formation. MO injection is followed by Alexa488 fluorescence (B, C). Once fixed at desired stage, embryos are analyzed by whole mount in situ hybridization (WISH) for the vascular markers msr (D, E), flk1 (F, G), erg (H, I) or VE-cadherin (J, K). Image published in: Xu K et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xtr Wt + rasip1 MO + NF20-37/38 (in situ hybridization) fig.S8 Anatomical Phenotype abnormal development of blood vessel Xtr Wt + rasip1 MO + NF20-21 (in situ hybridization) fig.S8.e Expression Phenotype increased amount aplnr expression in intermediate mesoderm Xtr Wt + rasip1 MO + NF25-27 (in situ hybridization) fig.S8.g Expression Phenotype decreased amount kdr expression in intermediate mesoderm Xtr Wt + rasip1 MO + NF33/34-35 and 36 (in situ hybridization) fig.S8.i Anatomical Phenotype abnormal trunk vasculature morphology Expression Phenotype decreased amount erg expression in intersomitic vessel decreased amount erg expression in posterior cardinal vein Xtr Wt + rasip1 MO + NF33/34-35 and 36 (in situ hybridization) fig.S8.k Anatomical Phenotype abnormal trunk vasculature morphology Expression Phenotype decreased amount cdh5 expression in intersomitic vessel decreased amount cdh5 expression in posterior cardinal vein Larger Image Printer Friendly View

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