Xenbase Image ID: 159017

Fig. 2. Vgll3 knockdown or overexpression impair trigeminal placode and nerve formation. (A) Embryos injected with v3MO or v3MOsplicing (v3LMOsp and v3SMOsp, 20 ng each) exhibit a reduced expression of islet1, neuroD and N-tubulin in the trigeminal placodes (arrowhead). (B) Overexpression of increasing amounts of vgll3 mRNA reduces islet1 and N-tubulin expression in stage 19 embryos. Double ISH shows no prominent overlapping staining between vgll3 (red) and N-tubulin (blue) (arrow). Injected side (*) was traced by lacZ staining. Gene expression were assayed by ISH. Arrowhead indicates the trigeminal placodes. (C) Quantification of results. Images on the top of bars indicated v3LMOsp defects rescued with increasing amounts of vgll3 mRNA injections. Three independent experiments were performed. The number of embryos analysed is indicated on the top of each bar. Views are dorsal-anterior excepted for lateral views for stage 28 embryos. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue isl1.S islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 18 preplacodal ectoderm neural crest isl1.S islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 19 preplacodal ectoderm neural crest neurod1.L neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. laevis Throughout NF stage 18 preplacodal ectoderm neuroectoderm neurod1.L neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. laevis Throughout NF stage 19 preplacodal ectoderm neuroectoderm tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 19 neuroectoderm neural plate preplacodal ectoderm tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 20 preplacodal ectoderm neural plate neuroectoderm tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 28 trigeminal ganglion nerve trigeminal placode brain central nervous system vgll3.L LOC108709205 X. laevis Throughout NF stage 19 presumptive rhombomere neuroectoderm neural plate Image source: Published Larger Image Printer Friendly View

Return to previous page