XB-IMG-159020
Xenbase Image ID: 159020
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Fig. 5. Vgll3 regulates CNC migration. CNC from neurula embryos injected with
tracer gfp mRNA and v3MO (40 ng or cMO) or vgll3 mRNA (1ng) were (A) grafted on
wild type embryos at stage 17 and migratory phenotype was analysed by GFP
fluorescence 18 hours after transplantation (quantification of results in the right
panel, inside panels showed GFP-positive grafted cells just after transplantation;
magnification has been adjusted to reduce the size of original images) or (B) were
plated on fibronectin and analysed 3h (a,d,g,j) and 18h (b,e,h,k) after plating.
Enlarged views of squares (c,f,i,l). Scale bars: 1 mm (j-k); 250 μm (l). (C) Ratio of
spreading measured by comparing the relative surface area between 18h and 3h of
culture (indicated by the line plot in a-k). #, P value <0.05; error bars represent
mean±s.e.m. (D) MyosinX expression in stage 16 (dorsal-anterior view) or stage 28
(lateral view) embryos. (*), injected side. Dotted line indicates midline embryo. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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