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Fig. 7. Vgll3 interacts with tead proteins and can activate pax3 independently of tead. (A) Embryos injected with vgll3 (1ng or lacZ) mRNA and T1MO/T2MO or cMO were fixed at stage 10 or 13 and analysed for pax3. Vgll3 induces ectopic pax3 expression when tead1 and tead2 have been knocked down. Lower right panel (IB myc): embryos were injected with 50 pg of myc-tead1 or myc-tead2 mRNAs with 15 ng of T1MO or T2MO and analysed by IB. T1MO and T2MO block efficiently tead1 and tead2 expression respectively. Erk2 is used as control. ni: non injected embryo. (B) Embryos injected with HA-vgll3, myc-tead1 or myc-tead2 mRNAs were processed for immunoprecipitation with HA (IP HA) or myc antibodies (IP myc) followed by IB with antibodies. Vgll3 interacts with tead1 and tead2 in the embryo. Lysate, IB of injected embryo before immunoprecipitation. Vertical white lines indicate spliced images in order to remove results not described in this paper (IP myc). Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 10 neuroectoderm pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 13 neuroectoderm neural plate border Image source: Published Larger Image Printer Friendly View

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