XB-IMG-159023
Xenbase Image ID: 159023
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Fig. 8. Vgll3 interacts with ets1 and activates a tead-dependent luciferase
reporter gene. (A) Pax3 expression in embryos injected with vgll3 or ets1 mRNA.
Ets1 overexpression induces ectopic pax3 expression likewise co-expression of ets1
and vgll3. (*), injected side. (B) Embryos were injected with myc-ets1 or HA-vgll3
mRNAs. Immunoprecipitation with myc (IP myc) or HA antibodies (IP HA) was
followed by IB with antibodies. (C) Schematic representation of the pGL284LUC
reporter plasmid used in transfection assay. The different cis-sequences are
depicted together with the MCAT cis-sequence (black box). (D) Left panel: ets1,
tead1 and vgll3 are expressed in HEK293 cells when analysed by RT-PCR. (-), no
cDNA. Right panel: HEK293 cells were transfected with HA-vgll3, HA-V3δTDU, myctead1
or myc-ets1 plasmids and similar amounts of proteins were checked by IB with
myc and HA antibodies. Gapdh and erk2 are used as control. (E) HEK293 cells were
co-transfected with pGL284LUC and HA-vgll3, HA-V3δTDU, myc-tead1 and mycets1
plasmids. Relative luciferase activity is expressed as x-fold luciferase activity compared to tead1 or ets1 alone. Bars show mean±s.e.m. of three independent
experiments carried out in duplicate. *P value <0.05. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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