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Figure S2. In vivo specificity of vgll3 morpholinos. Embryos were injected with 500pg of vgll3, vgll3 mismatch (mis) or vgll2 mRNAs in the presence (+) or not (-) of 40 ng of vgll3 (v3MO) or control (cMO) morpholino oligonucleotide antisenses. Stage 15 embryos were stopped and analysed by immunoblotting with HA antibody to detect vgll3. v3MO efficiently blocks the translation of vgll3 mRNA but has no effect on vgll3 mismatch mRNA neither on vgll2 mRNA. Erk2 is used for loading controls. Right panel, control MO (cMO) has no effect on vgll3 mRNA translation in an in vitro translation assay. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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