XB-IMG-159027
Xenbase Image ID: 159027
|
Figure S3. In vivo specificity and efficiency of v3MOsplicing. (A) Schematic
representation of the vgll3 locus. There are two vgll3 homeolog genes, vgll3.L and
vgll3.S, that are expressed in Xenopus laevis. Specific primers for vgll3.L and vgll3.S
(v3Lsp F and v3Ssp F respectively) and common primer for both mRNAs (v3sp R)
were chosen to detect normal and altered splicing for vgll3.L and vgll3.S mRNAs. The
position of the splicing blocking MO specific to vgll3.L pre-mRNA (v3LMOsp for
v3LMOe2i2) or to vgll3.S pre-mRNA (v3SMOsp for v3SMOi1e2) is indicated by a black
box. (B) Embryos were co-injected with 10, 20 or 40 ng of each morpholino splicing
(v3MOsplicing) and gfp mRNA as lineage tracer. Stage 15 embryos were analysed by
RT-PCR to detect vgll3 expression. V3MOsplicing decreases normal spliced vgll3.L
RNA or vgll3.S level compared to non-injected embryos in a dose-dependent manner.
PCR fragments corresponding to the altered splicing mRNAs appeared at expected
size for both vgll3 mRNAs. Odc is used for loading controls.-RT, no DNA for each
condition. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |