Xenbase Image ID: 159028

Figure S4. Dose dependent effect of v3MO splicing on N-tubulin and snail2 expression domain. (A) Embryos injected with v3LMOsp, specific to vgll3 form L or v3SMOsp, specific to vgll3 form S or both (v3MOsplicing) exhibit a reduced expression of N-tubulin in the trigeminal placodes (arrowhead) and an inhibition of CNC migration as shown snail2 expression. Stronger effect was observed when both splicing MOs were injected at 10ng each compared to single MO. Injected side (*) was traced by lacZ staining. Gene expression was assayed by ISH. Arrowhead indicates the trigeminal placodes. (B) Quantification of results. Three independent experiments were performed. The number of embryos analysed is indicated on the top of each bar. All views are dorsal-anterior. sp, splicing. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 20 neuroectoderm neural plate preplacodal ectoderm tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 21 neuroectoderm neural plate preplacodal ectoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 19 neuroectoderm neural plate neural crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 20 neuroectoderm neural crest neural plate cranial neural crest Image source: Published Larger Image Printer Friendly View

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