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Figure S5. Vgll3 impairs trigeminal nerve formation. (A) Embryos injected with 40 ng of v3MO exhibit a decrease of HNK-1 staining (arrowhead) and a quivering phenotype for 3A10 staining (bottom panel) in the trigeminal ophthalmic and maxillomandibular branches (50%, n=30 for both staining). (B) Embryos injected with vgll3 mRNA (1ng) exhibit a similar decrease of staining and a quivering phenotype in the trigeminal branches for HNK-1 (50%, n=25) and 3A10 staining (80%, n=25). Major branch connected to trigeminal ganglia disappeared (arrowhead) (C) Upper row: Embryos injected with cMO or lacZ mRNA show no difference in HNK-1 staining between non injected side (ni) or injected side (*). Lower row: Stage 14 embryos injected with cMO, v3MO, gfp or vgll3 mRNAs show no apoptotic cells when analysed by TUNEL. The median line of the embryo is indicated with a dotted line. The injected side was traced by lacZ staining. HNK1 (specifically labelling axonal outgrowth antibody) and 3A10 (neurofilament specific antibody) expression was assayed by immunohistochemistry on stage 28 and stage 42 respectively. (D) TUNEL apoptosis analysis of stage 17 embryos. V, trigeminal nerve; VII, facial nerve; ey, eye; ov, otic vesicle. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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