Xenbase Image ID: 159030

Figure S6. A second vgll3 morpholino (v3MO2) phenocopies v3MO effects. (A) Embryos were injected with 10 ng of a second non overlapping vgll3 (v3MO2) morpholino oligonucleotide antisense, then fixed at stage 16 or 19 and stained for snail2 expression. No significant effect was detected at stage 16 but defects in neural crest migration were observed at stage 19 (brackets). (*) injected side. (B) Specificity of v3MO2 in in vitro translation experiments. (C) Immunohistochemistry analysis of HNK-1 and 3A10 (stage 28 and stage 35 respectively). White and black arrowheads show defects of the ophthalmic and maxillo-mandibular branches of the trigeminal nerve respectively. (*) injected side. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 16 neuroectoderm neural crest chordal neural plate border neural plate border snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 19 neuroectoderm neural crest cranial neural crest neural plate neural plate border Image source: Published Larger Image Printer Friendly View

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