Xenbase Image ID: 159032

Figure S8. The histidine repeat in vgll3 is required for pax3 activation. (A) Sequence alignment of histidine (H) tract in Vgll3 proteins from different vertebrate species. Numbers indicate the relative position of the histidine tract. Xenlae, Xenopus laevis, Xentrop, Xenopus tropicalis. (B) Embryos were injected with 1 ng of vgll3 (vgll3 wt) or vgll3Dhis, fixed at stage 11 and analysed for pax3 expression. Vgll3Dhis cannot activate pax3 (100%, n=30). (*) injected side. (C) HEK293 cells, recently tested for contamination, were transfected with 1μg of plasmid encoding HA-vgll3wt or HAvgll3Dhis, fixed and analysed by immunocytochemistry using HA antibody. The deletion of histidine tract from vgll3 does not impair its nuclear localization. White arrow indicates nuclear staining. Scale bars, 10 μm. Image published in: Simon E et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 11 neuroectoderm Image source: Published Larger Image Printer Friendly View

Return to previous page