XB-IMG-159094
Xenbase Image ID: 159094
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Figure 1. Xenopus n1-src elicits neurite-like processes in fibroblasts. A, Amino acid alignment of the N1-microexon in mammalian, Xenopus, and fish species. +, Basic; −, acidic; φ, hydrophobic amino acid sidechains. B, Representative COS7 cells cotransfected for 4 d with Src-FLAG and CFP constructs. Cells were stained for Src (anti-FLAG) and CFP. C, Quantification of process outgrowth in COS7 cells. Each process was defined as an extension longer than one cell soma diameter and <2 μm in diameter. Data are plotted as mean ± SEM (n = 3 independent experiments, analyzed by Kruskal–Wallis 2-tailed ANOVA; ***p < 0.001; Scale bar, 10 μm). Image published in: Lewis PA et al. (2017) Copyright © 2017 Lewis, Bradley et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |