Xenbase Image ID: 159121

Fig. 1. MO inhibition of myocardin expression results in reduced cardiac gene expression and defective cardiac morphogenesis. (A) Ventral view of embryo injected with control MO and then assayed by in situ hybridization for MHC expression. The paired heart patches are clearly visible. Injected side is indicated with an arrow. (B) Section through heart of embryo injected with control MO. Heart tissue has been stained for Nkx2–5 transcripts. At this stage the myocardium is a simple linear tube surrounding the endocardial precursor cells. (C) Ventral view of embryo injected with MO directed against myocardin and assayed for MHC expression. Note that no MHC expression is detected on the injected side of the embryo (arrow). (D) Section through heart of embryo injected with myocardin MO. Heart tissue has been stained for Nkx2–5 transcripts. Note that the morphology of the developing heart is disrupted on the injected side (arrow). Adapted from [27] with permission from Elsevier. Image published in: Warkman AS and Krieg PA (2007) Copyright © 2007. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + myocd MO + NF25-26 (in situ hybridization) Fig 1. C Expression Phenotype decreased amount myh6.L expression in primary heart field Xla Wt + myocd MO + NF25-26 (in situ hybridization) Fig 1. D Anatomical Phenotype abnormal endocardial tube morphology abnormal heart morphology Expression Phenotype decreased amount nkx2-5.L expression in heart Larger Image Printer Friendly View

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