Xenbase Image ID: 159123

Fig. 3. Mutational analysis of the ANF promoter using Xenopus transgenesis. (A) Alignment of the proximal promoter region of the human (top) and Xenopus (bottom) promoter sequences, showing conservation of sequence, spacing and orientation of transcription regulatory sequences. Two conserved sequences that do not correspond to binding sites for known transcription factors are indicated (Unknown 1 and 2). (B) Fluorescent image of the heart of Xenopus embryo expressing GFP reporter under control of the wild type ANF promoter. Note that GFP expression is restricted to the atrial chambers (a). (C) Fluorescent image of the heart of a Xenopus embryo expressing GFP under control of an ANF promoter in which the NKE (Nkx2–5 binding site) has been mutated. Note that mutation of the NKE prevents restriction of expression to the atria, with prominent expression persisting in the ventricular myocardium. Part A is adapted from [68] with permission from Elsevier, and Parts B and C are reprinted from [55] with permission from Elsevier. Image published in: Warkman AS and Krieg PA (2007) Copyright © 2007. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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