Xenbase Image ID: 159143

Figure 1. Brca2 and Rad51 Function in DNA Replication(A) The Xenopus Brca2 protein. See also Figures S1 and S2.(B) Brca2 and Rad51 immunoprecipitations (IPs) and western blots (WBs).(C) Brca2 and Rad51 depletion from egg extract.(D) Chromatin binding time course with or without recombinant geminin. NS, no sperm nuclei.(E) Brca2 and Rad51 chromatin binding normalized to histone H2B. Mean optical density ± SD of three experiments (n = 3) is shown.(F) Chromatin binding time course in mock and Brca2-depleted extracts.(G) Relative incorporation of α-32P-dCTP over time in mock and Brca2-depleted extracts. Counts per minute for mock-treated extracts at 150 min were considered as 100%.(H) DNA replication in extracts depleted as indicated and reconstituted with 50 ng/μL recombinant Brca2c or Brca2d proteins. L-APH (3 μM) or L-APH plus S1 nuclease (0.1 U/μL) was added at the start where indicated. Nuclei were pre-treated with MMS (1% v/v) or UV (1,000 J/m2) where indicated. Counts per minute in mock-treated extracts at 150 min were considered as 100%. Mean values ± SD (n = 3) are shown.See also Figures S1–S3. Image published in: Kolinjivadi AM et al. (2017) © 2017 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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