Xenbase Image ID: 159176

Figure 4: Localization of the asymmetric gene Xnr-1 (nodal) is not affected by HCN4- DN and ZD7288. (A) Representative images of approximately stage 21 embryos assayed for Xnr-1 (nodal) expression by in situ hybridization and quantification of area of nodal expression. Red dotted line is midline and L representing left-side, R representing right-side, H representing head and T representing tail of embryos. Scale bar = 0.25 mm. (i) is no probe (negative) untreated control, (ii) Control embryos with Xnr-1 signal – red arrow, (iii) ZD7288 treated (from stage 1-10) embryos with Xnr-1 signal – red arrow, (iv) HCN4-DN mRNA injected (in both blastomeres at 2-cell stage) embryos with Xnr-1 signal – red arrows, and (v) Quantification of area of Nodal expression in embryos showed no significant change in the area of Nodal expression in HCN4-DN mRNA injected and ZD7288 treated embryos. N>10, Data was analyzed by one-way ANOVA, n.s. – Non-significant. (B) Representative images of approximately stage 23 embryos assayed for Lefty expression by in situ hybridization and quantification of area of lefty expression. Red dotted line is midline and L representing left-side, R representing right-side, H representing head and T representing tail of embryos. Scale bar = 0.25 mm. (i) is no probe (negative) untreated control, (ii) Control embryos with Lefty signal – yellow arrow, (iii) ZD7288 treated (from stage 1-10) embryos with Lefty signal – yellow arrow, (iv) HCN4-DN mRNA injected (in both blastomeres at 2-cell stage) embryos with Lefty signal – yellow arrows, and (v) Quantification of area of Lefty expression in embryos showed no significant change in the area of Lefty expression in HCN4-DN mRNA injected and ZD7288 treated embryos. N=10, Data was analyzed by one-way ANOVA, n.s. – Non-significant. Image published in: Pai VP et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue nodal1.L nodal, nodal-related 1, nr1, nr1-a, spaw, Xnr-1, Xnr1 X. laevis Throughout NF stage 21 left lateral plate mesoderm lefty.L antivin, atv, leftb, lefty-1, lefty-a, lefty-b, lefty1, leftyb, XATV, Xlefty X. laevis Throughout NF stage 23 left lateral plate mesoderm Image source: Published Larger Image Printer Friendly View

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