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Figure S2: HCN4-DN mRNA depolarizes membrane voltage in Xenopus embryos in vivo. Fluorescence intensity measurements of CC2 -DMPE:DiBAC stained embryos extrapolated to estimate approximate membrane voltage values using electrophysiology measurements. N=7 for each experimental group. Neural measurements (red square) of controls are significantly hyperpolarized versus non-neural measurements (blue squares). Embryos injected with HCN4-DN mRNA in both blastomeres at 2 -cell stage show depolarization in the neural measurements. Data are represented as mean + S.E.M., ***p<0.001. A one -way ANOVA analysis was performed. Image published in: Pai VP et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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