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Figure S3: HCN2-DN mRNA injections in both blastomeres at 2-cell stage do not result in left-right phenotypes. Quantification of stage 45 tadpoles for left- right organ (Heart, Gut and Gallbladder) laterality with or without microinjecting HCN2-DN mRNA (~0.7 ng/injection) in both blastomeres at two -cell stage as indicated in the illustrations. No significant incidence of heterotaxia was observed when HCN2-DN mRNA was injected in both blastomeres at 2 - cell stage, in comparison to controls. The experiment was conducted in triplicates and data was pooled to run a χ2 analysis. Image published in: Pai VP et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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