Xenbase Image ID: 159183

Fig. 2. MO-mediated depletion of Rnf146 and its effects on Wnt signaling. (A) Western blotting assessment of the blocking effect of Rnf146 MO on translation of an rnf146-myc mRNA. β-Actin serves as a loading control. (B) Western blotting detection of Axin1 and β-catenin in cMO and/or Rnf146 MO injected embryos. 40–80 ng Rnf146 MO was injected into both cells at the 2-cell stage. 80 ng cMO was injected as a control for non-specific effects. Injected embryos were then collected at the stage 12 and subjected to western blotting analysis using antibodies against Axin1 or β-catenin. β-Actin serves as loading controls. (C) Results from TOPFlash dual-luciferase assay in cMO, Rnf146 MO-injected embryos and embryos injected with mRNA encoding Rnf146 (2 ng). cMO or Rnf146 MO in indicated doses together with 20 pg TOPFlash reporter DNA and 20 pg renilla luciferase DNA was injected into both cells at the 2-cell stage. Injected embryos were then harvested at stage 12 and subjected to Dual-Luciferase assay. Image published in: Zhu X et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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