Xenbase Image ID: 159186

Fig. 5. Functional interaction between maternal Rnf146 and Axin1. (A) Western blot detection of Axin1 and β-catenin. Embryos fertilized from oocytes injected with 6–10 ng AS3m were collected at the mid-blastula stage (st8) and subjected to western blotting analysis. (B) qPCR analysis of expression of siamois and nodal3.1 in early gastrula stage embryos depleted of maternal Rnf146, Axin1 or both Rnf146 and Axin1. (C) Schematic summary of the function of Rnf146 in regulating Wnt signaling via controlling Axin levels. Panel a, the expression level of cytosolic β-catenin is balanced by normal expression levels of Axin, which is regulated by Rnf146 and the TNKS poly-ADP-ribosylase. Panel b, depletion of Rnf146 results in more Axin to be stabilized, leading to reduced expression of β-catenin. Image published in: Zhu X et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page