XB-IMG-159211
Xenbase Image ID: 159211
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Figure 1. il-11 is expressed at the tip of blastema during tail regeneration. a, b Expression levels of il-11 were measured by qRT-PCR using RNA extracted from ~20 tadpoles. Tail stump tissues cut at the level of 0.5 mm anterior from the amputated plane were used. Vertical axes represent relative expression levels of il-11 normalised by those of ef1α. Mean ± s.e.m. n = 4. ND: not detected. c–f WISH was performed using tadpoles fixed at 0 c, 24 d, 72 e, or 120 hpa f. Anterior is to the left and dorsal is up. Blue/purple colour represents signals for il-11 expression. Brown pigments are melanophores of the tadpoles. Scale bars: 500 μm. Numbers at the bottom corner indicate the total ratio of tadpoles showing the corresponding expression pattern from at least two batches. Note that il-11 was expressed at the blastema tip at 72 and 120 hpa (black arrowheads) Image published in: Tsujioka H et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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