XB-IMG-159213
Xenbase Image ID: 159213
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Figure 3. Rescue experiment of the phenotype of il-11 KD tadpoles. a Schematic drawing of a rescue construct. Yellow boxes represent cis-regulatory elements, and green boxes represent coding sequences. gRNA #1U.LS target site was inserted in the construct. In the control construct, acgfp was inserted instead of il-11-p2a peptide-acgfp. b Schematic drawing of knock-in and knockdown. Bars: introns, orange boxes: untranslated regions, blue boxes: coding sequences, dark grey arrows: gRNA target sequences. c Schematic drawing of a rescue experiment. d–j Representative images of tails of control cas9 mRNA-injected d, e, il-11 knockeddown acgfp-expressing f, g, or il-11 knockeddown il-11–expressing h, i tadpoles, and their regeneration length j are shown. k, Schematic drawing of a rescue experiment using tadpoles treated with or without doxycycline. l-x Representative images of tails of untreated cas9 mRNA-injected l, m, il-11 knockeddown acgfp-expressing n, o, or il-11 knockeddown il-11–expressing p, q tadpoles, or doxycycline-treated cas9 mRNA-injected r, s, il-11 knockeddown acgfp-expressing t, u, or il-11 knockeddown il-11–expressing v, w tadpoles and their regeneration length x are shown. Dark-field images d, f, h, l, n, p, r, t, v and GFP2-filtered images e, g, i, m, o, q, s, u, w are shown. Regeneration length was normalised by snout to vent length (SVL). Box plots are inserted in the panels. Bars in the boxes represent median, upper and lower limits of the boxes represent the first and third quartiles, and whiskers represent maximum and minimum values. Scale bars: 1 mm. Anterior is to the left, dorsal is up. Yellow broken lines indicate outline of the tails. White broken lines indicate amputated plane. *P < 0.05, Tukey−Kramer’s test Image published in: Tsujioka H et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |