Xenbase Image ID: 159215

Figure 5. il-11 induces or maintains tissue progenitor cells across cell lineages during tail regeneration. a Gene expression profile of il-11 KD #1 and cas9 tadpoles. Left panel: volcano plot. Fold-change of expression level of il-11 KD #1 tadpoles over control tadpoles measured by RNA-sequencing are plotted on horizontal axis in log2 scale, and adjusted P-values are plotted on vertical axis in –log10 scale. Genes with significant fold-changes (adjusted P < 0.05) are plotted in red. Points beyond the range are plotted on the edge of the graph. Representative undifferentiated markers are plotted in blue. Right panel: top 10 genes which showed highest upregulation in il-11 KD #1 are listed. Mouse homologues of genes indicated in orange are reported to be selectively expressed in skeletal muscle in mice (Supplementary Fig. 7). These genes correspond to orange plots in the left panel. b Fold-change of expression levels of representative undifferentiated markers measured by RNA-sequencing. Mean ± s.e.m. n = 3. *adjusted P < 0.05, Wald test. c–h, Localisation of undifferentiated markers. Representative WISH images for not c, f, dcx d, g, or runx1 e, h in control c–e or il-11 KD #1 tadpoles f–h are shown. Anterior is to the left, dorsal is up. Blue/purple colour represents signals for the genes. Brown pigments are melanophores of the tadpoles. Scale bars: 500 μm. Numbers at the bottom corner indicate the total ratio of tadpoles showing the corresponding expression pattern from two batches Image published in: Tsujioka H et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page