XB-IMG-159281
Xenbase Image ID: 159281
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Figure 1. CFTR domain topology, gating mechanism, and localization of target positions.(A) Domain topology of the CFTR cut-ΔR(D1370N) background construct. TMDs, gray; NBD1, blue; NBD2, green; intracellular loops containing coupling helices (CH1-4, from left to right), violet; target positions, colored circles. Red star denotes mutation D1370N. (B) Cartoon gating cycle of WT CFTR; and two-state equilibrium gating (red box) in saturating ATP of the background construct in which the D1370N mutation (red star) disrupts ATP hydrolysis (red cross). ATP, yellow circles; ADP, orange crescents, upper ATP binding site represents non-catalytic site 1, lower site represents catalytic site 2. (C–D) Target positions highlighted in colored spacefill on the cryoEM structure of dephosphorylated closed human CFTR (PDBID: 5UAK). (C) Full-length structure with all target positions shown, color coding as in A. Φ values for positions 1246, 348, and 275 are taken from (Sorum et al., 2015). (D) Only NBDs and coupling helices (CH1-4) shown, viewed from an angle orthogonal to the membrane. Dotted ellipses identify ATP sites 1 and 2. Image published in: Sorum B et al. (2017) © 2017, Sorum et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |