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Figure 2—figure supplement 2. Stimulation of open probability by 2'-deoxy-ATP (dATP) or N6-(2-phenylethyl)-dATP (P-dATP) facilitates counting channels for low-Po mutants.Inward currents of single cut-ΔR(D1370N) channels harbouring the indicated mutations. After several minutes of low-Po activity in 10 mM MgATP (green bars), exposure to 5 mM Mg-dATP or 25–50 μM Mg-P-dATP (red bars) robustly stimulated Po. A statistical test based on comparison of the observed opening rate and cumulative open time (Csanády et al., 2000) allowed high-confidence exclusion of the presence of a second active channel for each of the four patches shown (p<10−9, p<10−11, p=0.015, and p<10−14, respectively). Image published in: Sorum B et al. (2017) © 2017, Sorum et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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