XB-IMG-159284
Xenbase Image ID: 159284
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Figure 2—figure supplement 3. Burst analysis of single-channel recordings for position 555 mutants.(A, F) Inward currents of single cut-ΔR(D1370N) channels bearing mutation R555C (A) or R555Q (F) in 10 mM MgATP (green bars). (B–C, G–H), Closed (B, G) and open (C, H) dwell-time histograms (Sigworth and Sine, 1987) constructed from the events list of the records in (A, F); lower binning limit is 12 ms. Solid red lines are maximum likelihood fits of the dwell-times by a double- and a single-exponential distribution, respectively, with time constants (τ(1, 2)) and fractional amplitudes (a1, a2) printed in the panels. Dotted black lines in panels (B, G) depict the two components of the fit; vertical blue line marks the burst delimiter (tcrit) calculated using the method of (Magleby and Pallotta, 1983): this method ensures unbiased estimation of mean burst and interburst durations by equalizing the probabilities of misassigning interburst events as flickery closures and vice versa. (D–E), (I-J) Dwell-time histograms of reconstructed interburst (D, I) and burst (E, J) events following suppression of closed events shorter than tcrit. Solid red lines in D, I replot the longer component of the double-exponential fits in (B, G): note, some flickery closures remain uncancelled (events above red fit line, right of blue line), but an equal number of interburst closures is cancelled (lack of events below red fit line, left of blue line). Solid red lines in (E, J) are maximum likelihood fits of the burst durations by single-exponential distributions (τ, time constant). Image published in: Sorum B et al. (2017) © 2017, Sorum et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |