XB-IMG-159289
Xenbase Image ID: 159289
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Figure 4—figure supplement 2. Burst analysis of single-channel recordings for position 961 mutants.(A, F) Inward currents of single cut-ΔR(D1370N) channels bearing mutation M961Y (A) or M961E (F) in 10 mM MgATP (green bars). (B–C, G–H) Closed (B, G) and open (C, H) dwell-time histograms (Sigworth and Sine, 1987) constructed from the events list of the records in (A, F); lower binning limit is 12 ms. Solid red lines are maximum likelihood fits of the dwell-times by a double- and a single-exponential distribution, respectively, with time constants (τ(1, 2)) and fractional amplitudes (a1, a2) printed in the panels. Dotted black lines in panels (B, G) depict the two components of the fit; vertical blue line marks the burst delimiter (tcrit) calculated using the method of (Magleby and Pallotta, 1983). (D–E, I–J), Dwell-time histograms of reconstructed interburst (D, I) and burst (E, J) events following suppression of closed events shorter than tcrit. Solid red lines in (D, I) replot the longer component of the double-exponential fits in (B, G). Solid red lines in (E, J) are maximum likelihood fits of the burst durations by single-exponential distributions (τ, time constant). Image published in: Sorum B et al. (2017) © 2017, Sorum et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |