Xenbase Image ID: 159295

Figure 3. Correlations between parameters of filopodial dynamics. (A) Properties of Xenopus RGC growth cone filopodia from a manually curated dataset of (n = 160 filopodia and N = 19 neurons). Distribution histograms are shown for selected parameters: maximum length reached during time-lapse, straightness at maximum length, median rate of tip extension during the time-lapse, median rate of base invasion during the time-lapse, proportion of time spent in extending state, and tip persistence; see full description in Materials and methods. Descriptive statistics for these and all other parameters are in Table S1. (B) Comparison of the filopodia phenotype between two analyses of the same dataset: the manually curated analysis and fully automated batch analysis. For each parameter, the normalized boxplot represents the difference of the batch analysis relative to the manual analysis; black and gray vertical lines represent the median and interquartile range of the manually curated dataset for each parameter. Boxes and thick vertical lines represent the interquartile ranges and medians of each parameter for the fully automated (batch) dataset. Full descriptive statistics are in Table S1. (C) Correlation matrix between parameters describing filopodium shape and movement in a dataset of Xenopus RGC growth cone filopodia (n = 160) expressing GAP-RFP and mNeonGreen. Color scaling indicates the sign of correlation (blue to red for positive to negative); circle size indicates correlation strength. (D) Positive correlation between tip persistence of a filopodium and its maximum reached length. (E) Negative correlation between the median rate of tip extension of a filopodium and its proportion of time spent stalling. (F) Correlation matrix visualizing the correlations between the initial movement of the filopodial tips and bases (in the first 20 s after their initial appearance) with other filopodial properties in subsequent time points (after initial 20 s). IQR, interquartile range. Image published in: Urbančič V et al. (2017) © 2017 Urbančič et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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