Xenbase Image ID: 159303

Figure 5. A Side-to-Side Temperature Gradient Leads to Asynchronous MBT Entry (A) Snapshots of embryo at 23°C at three different time points after it has experienced a side-to-side temperature gradient (11°C–25°C) from 1:10 hpf to 5:45 hpf. Two regions of equal size have been selected on the previously cold and previously warm side. (B) Number of visible cells in the previously cold region (blue) and the previously warm region (red) as indicated in (A). The number of visible cells has been calculated by taking the initial number of cells and then increasing it by one every time a cell division is observed. The black lines show a smoothed fit using the “robust LOESS” (quadratic fit) option in MATLAB. (C) The average division rate of the visible cells as calculated from the fitted curves in (B). The time of maximal average division rate is taken as a measure for MBT onset. MBT is found to occur ∼60 min later in the previously cold region than in the previously warm region (with a SD of 16 min; 3 analyzed embryos). Image published in: Anderson GA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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