XB-IMG-159306
Xenbase Image ID: 159306
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Figure S2, Related to Figure 5. Evolution of the Xbra Phenotype
(A) Onset of Xbra expression in untreated embryos. Each time point is five images of the same stained embryo.
(B) Quantification of Xbra expression pattern after MBT. N.S. = No staining, A. = Asymmetric staining, S. = Symmetric staining. Each set of control embryos was collected from the same fertilization as the set of gradient-treated embryos below.
(C) Mock-treated control and desynchronized embryos. Temperature gradient treatment (initial 23°C followed by 11°C-25°C gradient from 1:10 hpf – 5:36 hpf) of embryos in the side-to-side direction from the one-cell stage to just before mid-blastula transition resulted in severely desynchronized embryos compared to controls.
(D) Mock-treated control and desynchronized embryos. Temperature gradient applied in the top to-bottom direction (initial 23°C followed by 18°C at the animal pole and 24°C at the vegetal pole from 1:10 hpf to 8:55 hpf) reversed the usual direction of the cell division wave and produced embryos with larger animal cells and smaller vegetal cells compared to controls.
(E) Side-to-side gradient-treated embryo stained for Xbra but not bleached. Pigmentation and Xbra staining are both visible. Image published in: Anderson GA et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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