XB-IMG-159357
Xenbase Image ID: 159357
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Figure S2, related to Figure 2. Angptl4 is expressed in Spemann organizer, notochord, somites and eye of Xenopus embryos.
(A) qRT-PCR analysis of angptl4 in X. tropicalis embryos at different developmental stages. Ornithine decarboxylase (odc) was used for normalization. (B) Left, gene Set Expression Analysis (GSEA, Subramanian et al., 2005) showing that a set of 107 X. laevis Spemann organizer-specific genes (Ding et al., 2017) strongly correlated with
genes from six dorsal-ventral libraries comprising 15,976 genes ranked according to their correlation with angptl4. Right panel, a set of 70 ventral lip-specific genes (identified in Ding et al., 2017) was inversely correlated with angptl4. This data mining confirms that angptl4 is
expressed in the Spemann organizer, the precursor of the notochord, at early gastrula (st.10.5).
(C) Whole-mount in situ hybridization of angptl4 mRNA in X. tropicalis embryos at the indicated stages. Tb, tailbud mesoderm; no, notochord; ey, eye; pn, pronephros; so, somites; vbi, ventral blood island.
(D, E) qRT-PCR analysis of angptl4 in X. tropicalis embryos at stage 11 that were injected with two different angptl4 antisense splice-site (Mo1 (D), Mo2 (E)) or control (co) Morpholinos (Mo). Two different primer pairs were used to analyze exon skipping of Mo1 or Mo2 respectively. Image published in: Kirsch N et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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