Xenbase Image ID: 159400

Figure 3 Pax-6 expression throughout retinal development. In all panels except (A), the vitreal surface of the retina is to the right. In all panels dorsal is up. (A) A sagittal section through a stage 14 embryo. Anterior is to the right. Note that the staining is in the neuroepithelial (Neu.) layer and not in the underlying mesoderm (Mes.). (B) A transverse section through a stage 28 optic cup. The arrow points to the presumptive lens epithelium, which expresses high levels of Pax-6 at this stage, while the arrowhead indicates the epidermis. Note that expression in the retinal neuroepithelium (NR) is essentially uniform at this stage. RPE retinal pigment epithelium. (C) A transverse section through a stage 33/34 retina. The intensity of the Pax-6 staining is decreasing in the outer nuclear layer (O). Inner nuclear layer I, ganglion cell layer G, lens Le. The ciliary marginal zone (CMZ) is outlined. (D) A transverse section through a stage 42 retina using DIC optics. The outer nuclear layer and the outer part of the inner nuclear layer (arrows) do not express Pax-6, nor does the CMZ (outlined). The lens at this stage has also turned Pax-6 off except in the most distal region. (E) A higher magnification of the boxed region in (D). The undifferentiated mitotic cells (arrowhead) of the CMZ (outlined in black) do not express Pax-6; neither do the photoreceptor cells of the outer nuclear layer (arrow). (F) Antibody labeling of Pax-6 in a stage 42 retina. The fluorescence is confined to the nuclei of the ganglion cells (G) and the inner nuclear layer (I). The outer part of the inner nuclear layer shows no Pax-6 staining (arrows); neither does the marginal zone (outlined in white). The fluorescence in the lens is nonspecific. Scale bar is 100 mm in panel (A), 50 mm in (B,C), and 20 mm in (D–F). Image published in: Hirsch N and Harris WA (1997) Copyright © 1997. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 14 neuroectoderm pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 28 eye primordium optic vesicle lens placode pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 33 and 34 retina eye eye primordium optic vesicle pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 42 retina retinal neural layer retinal inner nuclear layer retinal ganglion cell layer pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 42 retina retinal neural layer retinal inner nuclear layer retinal ganglion cell layer Image source: Published Larger Image Printer Friendly View

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