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Fig. 7. FoxI1e actively promotes ectoderm formation. Embryos were injected at the two-cell stage with SBMO and then at the four-cell stage with cmBMP7. Animal caps were excised at stage 7 and cultured to stage 14. (A,B) Epidermal cytokeratin and E-cadherin are expressed in uninjected (epidermal) animal caps. Loss of FoxI1e downregulates expression of these markers. cmBMP7 neuralizes these caps, so the epidermal markers are reduced. (C-E) The neural markers Sox-2, NCAM and NRP-1 are induced relative to controls in cmBMP7-injected (neural) caps. This induction requires the presence of FoxI1e. Therefore, FoxI1e is necessary for expression of both epidermal and neural genes. (F) Expression of the mesodermal marker Xbra at stage 11 in caps excised at stage 7. Whole embryo expression is shown as a reference. Image published in: Mir A et al. (2007) Copyright © 2007. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + foxi1 MO + animal cap explant + NF14 (RT-PCR) fig.7.a Expression Phenotype decreased amount krt12.4.L expression in animal cap Xla Wt + dnbmp7.1 + animal cap explant + NF14 (RT-PCR) fig.7.a Expression Phenotype decreased amount krt12.4.L expression in animal cap Xla Wt + dnbmp7.1 + foxi1 MO + animal cap explant + NF14 (RT-PCR) fig.7.a Expression Phenotype decreased amount krt12.4.L expression in animal cap Xla Wt + foxi1 MO + animal cap explant + NF14 (RT-PCR) fig.7.b Expression Phenotype decreased amount cdh1.L expression in animal cap Xla Wt + dnbmp7.1 + animal cap explant + NF14 (RT-PCR) fig.7.b Expression Phenotype decreased amount cdh1.L expression in animal cap Xla Wt + dnbmp7.1 + foxi1 MO + animal cap explant + NF14 (RT-PCR) fig.7.b Expression Phenotype decreased amount cdh1.L expression in animal cap Xla Wt + dnbmp7.1 + animal cap explant + NF14 (RT-PCR) fig.7.c Expression Phenotype increased amount sox2.S expression in animal cap Xla Wt + dnbmp7.1 + animal cap explant + NF14 (RT-PCR) fig.7.d Expression Phenotype increased amount ncam1.L expression in animal cap increased amount nrp1.L expression in animal cap Larger Image Printer Friendly View

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