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Supplementary Figure 4 Analyses of il-11 knocked-down tadpoles. a-e, Genotyping of tadpoles used in Fig. 2i. Genomic DNA was extracted from tail stump tissues cut at the level 0.5 mm anterior from the amputated plane after measurement of regeneration length, and sequences corresponding to il-11.L (a, d), il-11.S (b, e), or positive control ef1a (c) were PCR-amplified. Individual identification numbers are indicated on top. Sizes of DNA markers were (from bottom to top) 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, and 3000 bp. If the genomic region between two gRNA target sites is deleted, a band will be detected as indicated by the red arrowhead. Blue arrowheads correspond to wild-type sequences. Yellow asterisks: non-specific bands. f, g, Snout to vent length (SVL, f) and regeneration length (g) used for calculation in Fig. 2i are shown. h, Biological replicates for Fig. 2i are shown. Different batches of tadpoles were used in each experiment (Exp.). Box plots are inserted in the panels. Bars in the boxes represent median, upper and lower limits of the boxes represent the first and third quartiles, and whiskers represent maximum and minimum values. †P < 0.05, Student’s t-test. †P < 0.05, Dunnett’s test. NA: not analysed. Image published in: Tsujioka H et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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