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Supplementary Figure 9 Undifferentiated cells are rescued by forced expression of il-11 in il-11 KD tadpoles. ISH images for not (a-d) or dcx (e-h). cas9 mRNA injected (a, e), acgfp (b, d, f, h) or il-11 (c, g)-expressing il-11 KD tadpoles were fixed at 2 dpa, and sagittally sliced. Anterior is to the left, dorsal is up. Scale bars: 100 μm. Magenta/purple colour represents signals for the genes. Brown pigments are melanophores of the tadpoles. Signals for not were detected in the notochord bud (blue arrowheads). Signals for dcx were detected in muscle (red arrowheads) and spinal cord (white asterisks). Numbers at the bottom corner are the total ratio of tadpoles, indicating the corresponding expression from at least two batches. Green broken lines indicate spinal cord and spinal cord ampulla, and yellow broken lines indicate notochord and notochord bud. Inset: magnified view of boxed area. Image published in: Tsujioka H et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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