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Supplementary Figure 10 Gain of function analysis of il-11 for expression of undifferentiated markers. Biological replicates for Fig. 6h-j using tadpoles from a different batch. Expression levels of not (a), dcx (b), or runx1 (c) were measured by qRT-PCR. Four to six tadpoles were used in each lot. Vertical axes represent relative expression levels normalised by those of ef1α. Mean ± s.e.m. n=3. *P < 0.05, Student’s t-test. RT-PCR signal for not (a) or runx1 (c) was not detected in two or one samples in acgfp-expressing tails due to low expression levels. Image published in: Tsujioka H et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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