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Figure S1. Xotx5b, Xvsx1, and Xotx2 Expression in the CMZ
Comparison between Xotx5b, Xvsx1, and Xotx2 mRNA or protein detection (red) and BrdU-positive cells (green) in the CMZ of st. 42 retinas, after an 8-h BrdU pulse. Since this time of incorporation corresponds to the average cell cycle length of a late embryonic retinal progenitor (see Figure S5), the region of green-labeled cells reasonably excludes the majority of post-mitotic cells. From its most marginal aspect (M) towards the central side of the retina (C), the CMZ recapitulates the different stages of embryonic retinal neurogenesis [23], with more marginal cells earlier (less mature) than more central ones. Green arrows point at the central boundary of BrdU immunodetection, and red arrows indicate the marginal border of mRNA/protein detection. Whereas mRNA detection of the three genes always largely co-localizes with BrdU-labeled cells, none of the cells expressing detectable levels of the corresponding protein contains BrdU. These data indicate that Xotx5b, Xvsx1, and Xotx2 start to be translated at a measurable level in post-mitotic cells.
https://doi.org/10.1371/journal.pbio.0040272.sg001 Image published in: Decembrini S et al. (2006) Copyright: © 2006 Decembrini et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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