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Fig.S.3 Phenotype of Uch37 morphant. (a) Uch37 MO specifically inhibits the translation of C-terminally HA-tagged Uch37 mRNA (1ng), but not MO-resistant Uch37 (Re.Uch37) mRNA (1ng) and human Uch37 mRNA (1ng). Four-cell stage embryos were injected in animal blastomeres with indicated reagents. Embryos were cultured to stage 10.5 and then subjected to western blotting. (b) Phenotypes of Uch37- depleted embryos. Embryos were injected at the four-cell stage with the indicated reagents (40 ng Co MO; 40 ng Uch37 MO; 2ng Re.Uch37) ventrally (b, left) or dorsally (b, right) and cultured to tadpole stage. (c, d) Morphant phenotypes were tabulated. Image published in: Han W et al. (2017) Copyright © 2017, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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