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Fig. S.5 Uch37 promotes Wnt signaling downstream of ß-catenin destruction complex. (a) Result of axis duplication assay in Fig 1g. (b, c) RT-PCR analysis using animal cap explants. Embryos were animally injected at four-cell stage. Animal cap explants were isolated at stage 9 and cultured until stage 11. Injected reagents are as follows, Co MO (20ng), Uch37 MO (20ng), myc-Dvl2 mRNA (200pg), ß-catenin mRNA (25pg), Re.Uch37 (1ng). (d) Western blot analysis using animal cap explants. Embryos were animally injected at two-cell stage. Animal cap explants were dissected at stage 9 and cultured until stage 11. Injected reagents are as follows, Co MO (40ng), Uch37 MO (40ng), Wnt8 mRNA (20pg), myc-Uch37 mRNA (1ng). Image published in: Han W et al. (2017) Copyright © 2017, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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