Xenbase Image ID: 159573

Fig.S.6 Uch37 interacts with Tcf7 in nucleus of Xenopus gastrula embryo. (a) Fractionated lysates from Xenopus gastrula embryos were used as expression input for Co-IP analysis in Fig 1A. (b) Uch37 is co-localized with Tcf7 in nucleus. Four- cell stage embryos were animally co-injected with HA-Uch37 (1 ng) and myc-Tcf7 (50pg). Animal cap explants were isolated at stage 10 and fixed with 4% paraformaldehyde for 2h. HA-Uch37 and myc-Tcf7 were immunostained with anti-HA (mouse, santacruz; 2nd antibody, Alexa-488, invitrogen) and anti-myc antibodies (rabbit santacruz; 2nd antibody, Alexa-594, invitrogen). Scale bar (white line) indicates 50μm. (c) Co-IP assay using HEK293FT cells, HA-Uch37 and indicated myc-tagged truncated mutants of Tcf7 were transfected. Cell lysates were immunoprecipitated with anti-myc antibody. Truncated mutants of Tcf7 are depicted on the left. Image published in: Han W et al. (2017) Copyright © 2017, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page