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Fig.S.8 Uch37 MO specifically blocks Tcf7-mediated Wnt activity and Uch37 is required for Tcf7 binding to promoter of Vent2. (a) Axis duplication assay. Four- cell stage embryos were injected in one ventrovegetal blastomere with indicated reagents (400pg Tcf7 mRNA; 400pg Lef1; 40ng Co MO; 40ng Uch37 MO). (b) Quantified result of a. (c) ChIP assay using Xenopus embryos (stage 11). 70 embryos were injected at two-cell stage as indicated (25pg myc-Tcf7 mRNA; 1ng wild type of Uch37 (WT); 1ng catalytically inactive Uch37 (IN); 40ng Co MO; 40ng Uch37 MO). Lysates were precipitated with anti-myc antibody. Precipitated Wnt target DNAs were analyzed by PCR. EF1α was used as a control for specificity. Image published in: Han W et al. (2017) Copyright © 2017, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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