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S4 Fig. Nonnegative matrix factorization (NMF)-predicted expression for genes most cor- related to principal component analysis (PCA) axes 1 and 2. Dissected regions are projected along PCA components 1 and 2 in a pattern matching with embryonic dorsal-ventral (D-V) and anterior-posterior (A-P) axes, respectively. We have selected the 5 genes most correlated to these components (from list in S4 Table) and predicted their expression pattern using NMF. Patterns for the 5 genes with best positive or negative correlation are presented. This indepen- dent analysis confirms that the selected genes show clear D-V or A-P pattern restrictions and could thus be good diagnostic markers for position along each axis, respectively. Image published in: Plouhinec JL et al. (2017) © 2017 Plouhinec et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |